Characterization of Mycobacterium smegmatis bacteriophage Ravenclaw - Free Essay Example

Sample details Pages: 4 Words: 1206 Downloads: 1 Date added: 2019/03/18 Category Analytics Essay Level High school Tags: Characterization Essay Did you like this example? To start off made sure the surface was cleaned with 1% ethanol, and the Bunsen burner must be turned on. Everything performed to isolate a phage must be done in an aseptic zone, in this case it was near the Bunsen burner. 5 ml of the soil sample was gathered near WSU campus and poured into a bioreactor which already included the enriched medium and .5 ml of the M. foliorum then sat on the tube shaker for seven days. Once the soil sample was on the shaker for a minimum of 24 hours, 1 ml of the soil mixture was transferred into a microfuge tube. The soil mixture spun in a microcentrifuge at speed for thirty seconds. Next, with a syringe with its plunger removed, a filter was attached to the top and transferred roughly half of the centrifuge mixture into it. The plunger was reinserted into the syringe, a filter the medium into a microfuge tube. The filtered medium in the microfuge tube was used as enriched lysate. Don’t waste time! Our writers will create an original "Characterization of Mycobacterium smegmatis bacteriophage Ravenclaw" essay for you Create order Afterward the enriched lysate was prepared, a plaque assay was performed by adding 50 ? µl of the enriched lysate into a tube of .5 of M. foliorum and letting it incubate for ten minutes. Meanwhile the enriched lysate mix was incubated for ten minute, 20 ? µl of CaCl2 was mixed with liquid top agar. Once ten minutes had passed, add the top agar mix into the phage lysate mix and swirl. The mixture of M. foliorum, phage lysate, and a separate tub of liquid top agar, it was poured into a new l-agar plate it was then incubated at 30? °C for seven days. With a growth of a phage on the plate, a spot test was performed in aseptic conditions. A grid was drawn on the plastic side of a new l-agar plate and labeled as a negative control. A tube of liquid top agar is added 20 ? µl of CaCl? ¬? ¬2 and that mixture was then mixed into a tube of M. foliorum. The mixture of CaCl2, liquid top agar, and M. foliorum was drained onto a new l-agar plate. As it becomes solid on the plate, 10 â€Å" 25 ? µl of the soil sample was transferred onto the grids that were drawn on the bottom of the plate. The l-agar plates with the mixture topped off are places in a 30? °C incubator for a week. As the lawn was visible, a plaque streak was performed by first choosing a plaque from the plate and touching the plaque with a sterile wooden pick that was then aseptically streaked onto a new agar plate. This process was done by using a sterile wooden stick, starting at the edge of the past streak for a three separate streak where an x was noticeable. Then a new layer of agar that was mixed with M. foliorum and CaCl2 was poured onto the plate where the phage was streaked. The streaked plate was incubated at 30? °C for a week to let the bacteriophage grow. After the streaked plate had been incubated at 30 for seven days, the next step was to devise the phage specimen located the plaque that was circled. A clean applicator was used to transfer the most isolated phage from the plate into a bioreactor that also included .5 ml of M. foliorum and 25 mL of the enriched medium and sat on the shaker for seven days. After the enriched culture had been on the shaker for seven days, the next step was to collect the lysate and filter-sterilize. A .22 ? µm filtered syringe disbursed the now filtered enrich medium into three separate labeled microfuge tubes. Determined the titer of the HTL by ten labeled microfuge tubes from -1 to -10. Each tube was mixed with 90 ? µm of phage buffer as well as 10 ? µm of the concentrated HTL to the -1 microfuge tube. Mixed 10 ? µm from the -1 tube into the -2 tube and continue transferring 10 ? µm from the previous tube into a new tube until reaching the -10 tube with a new tip each time transferring into a new tube. A negative control was needed, so the 11th tube was infected with M. foliorum and 10 ? µm of phage buffer and letting it sit for ten minutes. As ten minutes passed, all the plates were topped off with a mixture of liquid top agar and CaCl2 and labeled appropriately. Again, it they solidified and were moved into a 30 degree Celsius incubator for the next seven days After the plates were incubated at thirty degree Celsius for seven days, the next step was to degrade bacterial DNA / RNA in high titer lysate. First step was to disburse one mL of high titer lysate into a microfuge tube, then with gloves on, relocate to the designated nucleus work station. Added 5 ? µl of the nucleus mix into my sample, mixed by repeating, incubated at 37 degrees Celsius for ten minutes. After ten minutes of incubation, the second step was to denature the protein capsid to excrete the phage DNA. 500 ? µl of the nuclease HTL mixture was discharged onto two separate microfuge tubes with clean up resin. The next protocol was to isolate the phage genomic DNA by attaining two DNA columns, labeled with personal initials. Columns were attached to two three mL syringes, and 1.5 mL of the phage mixture were filtered into new microfuge tubes. Put column in a new microfuge tube by first unscrewing the column from the syringe before freeing the plunger. Afterwards, withdraw the plunger from the syringe barrel and screw back the column, Following the protocol, the next step was to rinse the salt from the DNA by using two syringe barrels filled with two mL of 80% isopropanol. Then repeat steps of isolating phage genomic DNA to have done three isopropanol washes. Afterwards, isopropanol residue was left and to remove it the columns were placed in a microfuge tube and spun at 10,000 x for a maximum of five minutes. Then, the columns were placed on a block that had been heated to 80o C for one minute to evaporate any excess isopropanol Eluted the DNA by incubating the columns at room temperature with 50 ? µl of ddH2O for one minute. After incubation, the samples spun again at 10,00 x for sixty seconds. The eluted phage DNA was gathered by incorporated the products from both microfuge tubes into one final tube. First started by mildly stirring the DNA specimen by finger vertexing and with the concentrated DNA sample calculate the amount of DNA sample. Next, made sets of restriction enzyme digest reaction by getting a tube of dH2O 100 ? µg, 10X reaction buffer 20 ? µl, 3 ? µl BamHI Enzyme, 3 ? µl EcoRI Enzyme, HindIII. Reactions were set up in the format the protocol was told as. There were four reaction tubes, three restriction enzyme digest and a controlled tube with no enzyme. To indicate the reaction, started by placing the reaction tubes in tube racks and placing them in the incubator ay 37 o and my TA removed them afterwards. Placed the 24 ? µl of reaction inside the wells of an agarose gel that was inside of the gel electrophoresis. TA provided a portion of kb ladder, dispersed all 10 ? µl of the ladder onto the gel in the lanes. After forty minutes, relocated the gel into a ziplock bag, and into a transilluminator where the DNA could be seen.

Creating a Healthy Environment for Your Prenatal Child...

Creating a healthy environment is critical for any baby’s well-being and healthy transition into this world. Teratogens are a main concern when creating a healthy atmosphere. For example, when my mother was pregnant with my brother, she did not understand the adverse effects on her baby’s development that smoking would create. Smoking was just routine for her, and the labels on the cigarettes as a fair warning didn’t change her opinion on smoking during her pregnancy. My father smoked as well. Even worse, a lot of the smoking occurred in the household, so smoke was always in the house and my mom was always breathing it in. Months went by during my mother’s pregnancy with her first child. Everything seemed to be going smoothly. Her baby†¦show more content†¦It can create a disorder known as Fetal alcohol spectrum disorder (FASD), which creates abnormalities such as facial deformities, defective face, limbs, and heart, learning disorders, and low in telligence. Even small amounts of alcohol can cause early pregnancy loss. Caffeine is also a teratogen that is easily accessible in sodas, coffee, teas, energy drinks, and even some chocolate. Doctors suggest being cautious when consuming small amounts of caffeine or even cutting it out of the prenatal diet completely. Caffeine seems to be the least harmful of the listed teratogens, as high amounts do not increase the risk of miscarriage and growth retardation. Both Cocaine and Meth are extremely harmful stimulants to prenatal children. Cocaine use during pregnancy lowers birth weight, head circumference, and length of the baby. Babies can be born with lower arousal, higher excitability, and lower quality of reflexes as well. Growing up, the baby can experience weakened motor development as well as a slower growth rate. Learning disabilities are also common, as well as behavioral problems. Marijuana also has negative consequences for children such as lower intelligence and an increa sed risk for the children using marijuana themselves by the age of 14. It is recommended that pregnant women do not use marijuana during their term or use any of the teratogens. Synergistic effects – that is, using or being around multiple teratogens is extremelyShow MoreRelatedThe Transformation Of Human Development1431 Words   |  6 Pagesuniversal and unique characteristics of each human (Berger, 48) The universal characteristics that each unit of life contains molecules that are full of information and instructions called DNA. Human development begins from zygote to newborn in which â€Å"prenatal development is divided into three categories: Germinal period, Embryonic period, and fetal period.† (Berger, 58) The Germinal Period appears begins during the first 2 weeks after conception. â€Å"The zygote begins duplication and division,† in whichRead MoreEffects Of Teenage Pregnancy On Children1625 Words   |  7 Pageswill be explained and evaluated by examining how support plays a role in the wellbeing of the child and mother. Many would say that having a child is a blessing, but not when you have a child at a young age. Teen pregnancy/motherhood have been greatly seen as an issue in society. â€Å"Research suggests that many associate teen mothers with welfare dependency, irresponsibility, stupidity, ignorance, laziness, child abuse, immorality, and promiscuity† (Eshbaugh, 2011). Many research has demonstrated the negativeRead MoreThe Breakdown Of Continuum Care952 Words   |  4 Pagescomponents are prenatal care, behavioral illness, newborn care, preventive care, healthy lifestyle, acute illness acute injury and rehab. The main challenge would be the improving of health care and managing any health care problems that might come up. A lot of the process as to do with the environment the patient lives in(In Information Infrastructure for Healthcare INFORMATION INFRASTRUCTURE FOR HEALTHCARE.). All this depends on the patient, do they want treatment, do they want to stay healthy and doRead MoreThe Is A Possible Future Effect Or Result? Essay1694 Words   |  7 Pagesseries of events or molecular functions ) The goal of PGD is to produce a viable and healthy child free of any severe hereditary diseases. â€Å"the issue here is whether this goal should include accepting embryos with a ‘carrier’ test result.† This means the embryos may be free of the defect with little possibility of them possessing the disease, however there is a possibility that their offspring (embryos child) may inherit the relevant genes. Therefore parents selecting purely unaffected and non-carrierRead MoreThe Definition Of Nurse Midwives1698 Words   |  7 Pagestaking place in the body to adjust to the fetus ongoing needs. The mother has numerous responsibilities such as nutrition, exercise, and bodily care to improve the conditions in which the baby is leaving in. Prenatal care is tremendously vital because it ensure that you and your baby are healthy as possible throughout the pregnancy. A number of factors affect the circumstances surrounding a pregnancy such as: medical conditions, previou s complicated deliveries, previous successful or unsuccessful pregnanciesRead MoreThe Policy Of Healthy Families America2205 Words   |  9 PagesHealthy Families America is a program created in 1992 by the Prevent Child Abuse America Program. The Ronald McDonald foundation contributes by founding to the program. Their mission is â€Å"to work with families who may have histories of trauma, intimate partner violence, mental health and or substance abuse issues† (Berger, 2000). Their goals are to enhance positive parent-child relationships, better the health of children, and prevent child abuse and neglect. HFA hope that by the end of the programRead MoreCaloric Intake and Childhood Obesity Essay2769 Words   |  12 PagesSince I was a child, I have always known I wanted to become a doctor, but I did not know what kind of doctor I wanted to become. Did I want to become a doctor to earn a lot of money and live a prosperous life, be respected in society, or so that I could sim ply help other people? The answer came to me not too long ago while I was volunteering at the Methodist Richardson Hospital. During my time in the children’s ward reading books with these children or even just talking to them, I felt a sense ofRead MoreThe Case Of Ta Esha1344 Words   |  6 Pageshospitalized until delivering. The doctor blamed formaldehyde in her family’s FEMA-funded trailer, their home since Hurricane Katrina displaced them from New Orleans. He also sternly said that Ronita stopped smoking, waited for few years, and gotten prenatal care; Ta’esha would be bigger and healthier. Ronita’s grandmother, who has obesity, diabetes, and heart problems, support the family with her disability insurance, Ronita’s youngest brother, Donnell, has cerebral palsy and frequent seizures, andRead MorePed 212 (Foundations of Movement and Motor Activities) Entire Course1793 Words   |  8 Pagesthe elementary school level. Some examples of issues might be childhood obesity, budget constraints, family / home environment, nutrition, prenatal care, lack of medical care or other factors that either offer an advantage or disadvantage to a child’s motor development. Review several scholarly sources that offer information on the issue. Include short summary of the issue in your initial post. Also address the following questions: 1. What are the advantages and/or disadvantages of this issueRead MoreEugenics And The Eugenics Movement3686 Words   |  15 PagesIntroduction Human beings have always been interested in success, in making sure they survive, and creating new tools and technology to enable them to reach these goals. So it should come as no surprise that eugenics follows this same guideline in human history. A term originally coined by Francis Galton, first cousin to Charles Darwin, eugenics simply means that the â€Å"best people† in society with the most â€Å"healthy† â€Å"normal† genes should continue to reproduce and pass on their â€Å"goodness† to the next generation

Role of Falstaff in Henry IV, Part One Essay Example For Students

Role of Falstaff in Henry IV, Part One Essay Falstaffs Role in Henry IV, Part OneHenry IV, Part One, has always been one of the most popular of Shakespeares plays, maybe because of Falstaff. Much of the early criticism I found concentrated on Falstaff and so will I. This may begin in the eighteenth century with Samuel Johnson. For Johnson, the Prince is a young man of great abilities and violent passions, and Hotspur is a rugged soldier, but Falstaff, unimitated, unimitable Falstaff, how shall I describe thee? Thou compound of sense and vice . . . a character loaded with faults, and with faults which produce contempt . . . a thief, a glutton, a coward, and a boaster, always ready to cheat the weak and prey upon the poor; to terrify the timorous and insult the defenceless . . . his wit is not of the splendid or ambitious kind, but consists in easy escapes and sallies of levity yet he is stained with no enormous or sanguinary crimes, so that his licentiousness is not so offensive but that it may be borne for his mirth.Johnson mak es three assumptions in his reading of the play:1. That Falstaff is the kind of character who invites a moral judgment mainly that he can answer to the charge of being a coward. 2. That you (the reader) can detach Falstaffs frivolity from the play and it can exist for its own sake apart from the major theme of the drama. 3. That the play is really about the fate of the kingdom, and that you (the reader) do not connect Falstaffs scenes with the main action. This means that the play has no real unity. Starting with Johnsons first assumption, I do agree with this. Any discussion of Falstaff is bound to include a judgement about his moral character. Is he a coward, a thief, a glutton? No one can deny that he is in fact a glutton and a thief. A coward is debatable. I choose to think he is. He is self centered and cares only for his own profit and enjoyment. He will protect himself at all costs including playing possum if necessary to avoid injury. When he misuses the money intended to buy troops and weapons, he turns it into profit for himself. Once again, with no concern for anyone else, he potentially jeopardizes the troops, the battle and the kingdom with substandard men and materials while making money for himself. It makes the reader question, what kind of friend is he to Hal that he would misuse the trust that has been given him. All the easier for Hal to ultimately recognize that this is not the kind of person or people he wants to associate himself with, let alone approve of . Johnsons second assumption that you can detach Falstaffs frivolity from the real drama is in fact true, but what would you have left? A less interesting, less amusing drama with only one main plot. Falstaff is of paramount importance to the sub-plot dealing with Hals decision between continuing his carefree life style or maturing into the role he is destined to play as a respected prince and later king. This story would be pretty dull if Hal didnt have to choose between an entertaining life like Falstaffs or an honorable one as a gallant warrior and respected leader. Johnsons last assumption that the Falstaff scenes have nothing to do with the main action is incorrect if you agree that this sub-plot is necessary for an engaging drama. In Act 2, Scene 4, after Hal says, while role playing as the King with Falstaff, That villainous abominable misleader of youth, Falstaff, that old white-bearded Satan. Falstaff, as Hal, tries to reason, No, my good lord, banish Peto, banish Bardolph, banish Poins, but for sweet Jack Falstaff, kind Jack Falstaff, true Jack Falstaff, valiant Jack Falstaff, and therefore more valiant, being as he is old Jack Falstaff, banish not him thy Harrys company, banish him not thy Harrys company; banish plump Jack, and banish all the world. Hal, again as the King, says, I do, I will. He indicates that after becoming king he would choose to rid the kingdom of people the likes of Falstaff. He is indicating that he has chosen the path for his life and made his own moral judgement on Falstaff. This scene and therefore Falstaffs very being are significant to show Hals evolution into a true prince. .u5df06aa2e5b28c2d07db483d4205301b , .u5df06aa2e5b28c2d07db483d4205301b .postImageUrl , .u5df06aa2e5b28c2d07db483d4205301b .centered-text-area { min-height: 80px; position: relative; } .u5df06aa2e5b28c2d07db483d4205301b , .u5df06aa2e5b28c2d07db483d4205301b:hover , .u5df06aa2e5b28c2d07db483d4205301b:visited , .u5df06aa2e5b28c2d07db483d4205301b:active { border:0!important; } .u5df06aa2e5b28c2d07db483d4205301b .clearfix:after { content: ""; display: table; clear: both; } .u5df06aa2e5b28c2d07db483d4205301b { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u5df06aa2e5b28c2d07db483d4205301b:active , .u5df06aa2e5b28c2d07db483d4205301b:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u5df06aa2e5b28c2d07db483d4205301b .centered-text-area { width: 100%; position: relative ; } .u5df06aa2e5b28c2d07db483d4205301b .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u5df06aa2e5b28c2d07db483d4205301b .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u5df06aa2e5b28c2d07db483d4205301b .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u5df06aa2e5b28c2d07db483d4205301b:hover .ctaButton { background-color: #34495E!important; } .u5df06aa2e5b28c2d07db483d4205301b .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u5df06aa2e5b28c2d07db483d4205301b .u5df06aa2e5b28c2d07db483d4205301b-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u5df06aa2e5b28c2d07db483d4205301b:after { content: ""; display: block; clear: both; } READ: The Catcher In The Rye Essay SacrificeFalstaffs character is necessary to Hals character development just as Hotspurs temperament is necessary to his. Falstaffs wit, humor and amusing antics are needed to develop Hal. He helps us relate to Hal and his decision. We know people of all types of character and personality in our lives. They influence our thinking and decisions. So it is also necessary for Hal. Wether Falstaff is only a coward and glutton, or a person who has an amusing way of expressing his deeply felt personal and political beliefs is a matter of individual interpretation. I am not sure that it really matters as long as it contributes to Hals maturing process, and it does. In conclusion, every age of man has and will continue to judge Falstaffs role based on the morals and the thinking of the day. His frivolity is necessary to make the play amusing and interesting enough to hold the readers/viewers attention. However, that Falstaffs scenes are needed should go without question leaving the critics and us only to debate his motivation and his tactics. Category: English

Amistad free essay sample

The Amistad movie is about a group of West Africans captured as slaves to America. Led by Sengbe Pieh, who was renamed as Joseph Cinque by the Spaniards who bought his later decided to revolt against his captors. The movie started at the scene where cinque was trying to free himself from his chains. Him and his fellow slaves managed to get control of the ship by killing many of the cruise men living two of then alive to guide then back to Africa. They fought hard to stay alive, but unfortunately they found themselves in the strange waters of in America and charged with murder. Their fate lied in the hands of a real estate and property lawyer Roger Baldwin, who had to prove that these men are from Africa and were illegally stolen in to slavery. This would gain their freedom because African slavery was illegal. With the help of former president John Quincy Adams Cinque and his fellow Africans were set free. We will write a custom essay sample on Amistad or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Unfortunately these men will be going home to civil war zone. I was captured by the intense emotion from the beginning of the movie to the end of it. The movie depicts the inhumane acts of humans. The pain and suffering that these people went through is none like other. Yet they yearn for their home and they fought to get back to it. The ending of the film was discouraging. Cinque and the rest of the freed slaves were going back to a country at war. Cinque’s family was probably captured in to slavery and the hope of seeing his family will be an undisturbed reality for him. My reaction to the movie â€Å"Amistad† was of mixed emotions. Horror, anger, sadness, and a plethora of a other emotions ran through my mind as the movie went on. There were many important parts in the movie, the part where the slaves took over the ship, their capture and their torture on the ship, their trials, and the return to Africa. Each scene had a new emotion with it. The most important scene to the movie in my opinion is the scene in where the slave are kidnapped and brought over to America. As the movie started I tried to put myself in the slaves shoes as well as I possibly could. Before the slaves were capture their lives were as normal and as happy as yours or mine, but in an instant they were captured and thrown on a ship. Men, woman, and children were stripped of their clothes and thrown on a ship. Husbands had to watch their wives and children be thrown into little corridors and suffer. While they, themselves just tried to survive. Villages that were once united as friends were forced to turn against each other to survive. In order to get their freedom they must

Anaylsis between Thoreaus Civil Disobedience & Kings Letter essays

Anaylsis between Thoreau's Civil Disobedience & Kings Letter essays In Civil Disobedience and Letter from Birmingham Jail, Henry David Thoreau and Martin Luther King use different techniques to fully convey their own opinions on injustice in the government; Thoreau uses a metaphor while King uses an illustration in order to establish emotional appeals. Even though Civil Disobedience, by Henry David Thoreau, was written in 1849, and King was imprisoned in 1963, each recognize that injustice occurs in our government. Each authors techniques are effective in proving that injustice should be demolished. Henry David Thoreau compares the United States government to a political machine. This machine has friction, which is Thoreau explains is injustice. He states that If the injustice is part of the necessary friction of the machine of the government, let it go, let it go...certainly the machine will wear out (Thoreau 8). Thoreau is stating that this machine will eventually cease to run if the friction continues to occur. By having injustice in the government, corruption is ultimately the result, and the corrupt government will eventually crumble. Thoreau also explains that the people who are affected by the injustice should stop it, or at least make an effort to stop it. Let your life be a counter friction to stop the machine (Thoreau 8). Sitting back and letting others fix the problem will accomplish nothing, but making any effort towards correcting the injustice is an accomplishment in itself. Although Thoreaus metaphor established an emotional appeal effectively, Kings illustrations elucidate the issue of injustice equally as effectively. King makes mention of the Christians that existed during times of great persecution, and he admires the way that they stood up for what they believed in. King explains that Whenever the early Christians entered a town, the people in power b ...

What strategies do cyber security use in order to avoid the hackers Essay

What strategies do cyber security use in order to avoid the hackers prevailing users of e-commerce websites - Essay Example About 52% of the UK consumers use online shopping as an opportunity to save cash. In 2009, an estimated 608 million card payments were made online, with  £47.2 billion being spent in 2011 around 74% of UK homes have internet access (Government of the United Kingdom, 2011). The cyber security departments need to have strategies that balances resiliency with focused, protection, risk-informed prevention and preparedness activities in order to manage and reduce the most deleterious risks in e-commerce. E-commerce is regarded as the wave of the future. However, as organizations come up with new web-based strategies so as align themselves with the future commerce, problems are expected on the way inform of cyber attacks. Cyber security is at a sensitive stage in the evolution of e-commerce. A major attack at this particular time, if successful, could significantly affect the future development of the e-commerce business model. This could result to harsh ramifications for the victimized organization(s) in particular. The paper will highlight the strategies used by cyber security in order to avoid the hackers prevailing users of e-commerce websites. The ever increasing roles of information technology as well as the growth of the e-commerce sector have made cyber security be very important essential to the economy. The cyber security standards are prevailing security standards that enable various organizations to practice safe security techniques so as reduce the number of successful cyber security attacks (Amoroso, 2012). The misuse of the online environment through hacking, fake websites and other means have threatened to undermine the social and economic benefits of the online environment by eroding confidence and trust in the security and safety of the online environment (Shoemaker & Conklin, 2011). This is what has necessitated the e-commerce industry to come up with strategies that will boost customer confidence in engaging in online transactions. These

The E-Trade Baby Essay Example | Topics and Well Written Essays - 500 words

The E-Trade Baby - Essay Example In order to identify the rhetorical strengths and weaknesses of the E-Trade Baby commercials, their ethos, logos, and pathos should be examined. Ethos implies the personality and trustworthiness of the speaker associated with the argument. Ethos raises issues of ethics and confidence between the speaker and the audience. In terms of ethos, the E-Trade Baby ads are quite effective in drawing out the interest of the audience by using an ‘infantile’ personality that normally appeals to the emotion of audiences. In terms of integrity, the ads are also successful since the babies’ ‘voices’ are from trusted people in the finance and investment industry. The E-Trade Baby simply used the attractive and adorable personalities of the babies to convey its reliable messages about financing and investing. The ads successfully identified with their audiences and their argument. They also appeal to the sense of necessity of the audiences by building a whole new real m of investing, or also referred to as electronic trading. Logos denotes the application of numbers, statistics, reason, and logic. Quite frequently, logos appears concrete and material, far more tangible and ‘real’ compared to other rhetorical techniques that it does not appear an advertisement approach at all. In terms of logos, the E-Trade Baby ads are quite illogical since it is commonsensical knowledge that babies cannot talk in the way they are portrayed in the ads making the entire endeavor weak within the domain of logos. But in terms of the presentation of statistics and facts, the ads have been concise and straight to the point. Pathos appeals to the emotions of audiences. The E-Trade Baby ads try to appeal to the emotions of love, compassion, affection, sympathy, and happiness. They successfully used an emotional appeal by identifying with the sense of their audience. The ads did not abuse any ethical or emotional appeal since